High Production of Thermostable beta-Galactosidase of Bacillus stearothermophilus in Bacillus subtilis.

نویسندگان

  • H Hirata
  • S Negoro
  • H Okada
چکیده

By cloning the beta-galactosidase gene of Bacillus stearothermophilus IAM11001 (ATCC 8005) into Bacillus subtilis, enzyme production was enhanced 50 times. beta-Galactosidase could be purified to 80% homogeneity by incubating the cell extract of B. subtilis at 70 degrees C for 15 min, followed by centrifugation to remove the denatured proteins. Because of its heat stability and ease of production, beta-galactosidase is suitable for application in industrial processes.

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عنوان ژورنال:
  • Applied and environmental microbiology

دوره 49 6  شماره 

صفحات  -

تاریخ انتشار 1985